abstract：:Concern about the malicious applications of botulinum neurotoxin has highlighted the need for a new generation of safe and highly potent antitoxins. In this study, we developed and evaluated the preclinical pharmacology and safety of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A (BoNT/A). As an alter...

journal_title：Biochimie

authors： Yu YZ,Zhang SM,Wang WB,Du Y,Zhu HQ,Wang RL,Zhou XW,Lin JB,Wang S,Yu WY,Huang PT,Sun ZW

更新日期：2010-10-01 00:00:00

abstract：:GcvB is a non-coding RNA that regulates oppA mRNA in different bacterial species by binding a GcvB GU-rich region named R1 to oppA mRNA. A secondary putative interaction site (PS1) was identified in this study that is able to form a second nearly perfect 10 base-pair duplex between these two RNAs in Escherichia coli. ...

journal_title：Biochimie

authors： Busi F,Le Derout J,Cerciat M,Régnier P,Hajnsdorf E

更新日期：2010-10-01 00:00:00

abstract：:Over-expression of efflux transporter P-glycoprotein (PgP) encoded by ABCB1 gene has been implicated in poor responsive epilepsy. Several genetic variants have been shown to influence the expression levels of P-glycoprotein. The aim of the present study was to investigate the role of ABCB1 polymorphisms: C1236T, G2677...

journal_title：Biochimie

authors： Grover S,Bala K,Sharma S,Gourie-Devi M,Baghel R,Kaur H,Gupta M,Talwar P,Kukreti R

更新日期：2010-09-01 00:00:00

abstract：:The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...

journal_title：Biochimie

authors： Nüss D,Goettig P,Magler I,Denk U,Breitenbach M,Schneider PB,Brandstetter H,Simon-Nobbe B

更新日期：2010-08-01 00:00:00

abstract：:Here we describe a convenient and robust ligase-independent method for construction of combinatorial and random mutant libraries. The homologous genes flanked by plasmid-derived DNA sequences are fragmented, and the random fragments are reassembled in a self-priming polymerase reaction to obtain chimeric genes. The pr...

journal_title：Biochimie

authors： An Y,Wu W,Lv A

更新日期：2010-08-01 00:00:00

abstract：:Thorium-232 ((232)Th), a natural radionuclide from the actinide family, is abundantly present in monazite and other ores. It is used as one of the prime fuel materials in nuclear industry and may pose an exposure risk to nuclear workers and members of the public. Human erythrocytes, as a classical cellular membrane mo...

journal_title：Biochimie

authors： Kumar A,Ali M,Pandey BN,Hassan PA,Mishra KP

更新日期：2010-07-01 00:00:00

abstract：:The oncogenic potential of the high-risk human papillomavirus (HPV) relies on the expression of genes specifying the E7 and E6 proteins. To investigate further the variation in oligomeric structure that has been reported for different E7 proteins, an HPV-18 E7 cloned from a Hispanic woman with cervical intraepithelial...

journal_title：Biochimie

authors： Liu S,Tian Y,Greenaway FT,Sun MZ

更新日期：2010-07-01 00:00:00

abstract：:The eukaryotic ribosomal protein S15 is a key component of the decoding site in contrast to its prokaryotic counterpart, S19p, which is located away from the mRNA binding track on the ribosome. Here, we determined the oligopeptide of S15 neighboring the A site mRNA codon on the human 80S ribosome with the use of mRNA ...

journal_title：Biochimie

authors： Khairulina J,Graifer D,Bulygin K,Ven'yaminova A,Frolova L,Karpova G

更新日期：2010-07-01 00:00:00

abstract：:Prostaglandin E synthase (PGES), which converts cyclooxygenase (COX)-derived prostaglandin H(2) (PGH(2)) to PGE(2), is known to comprise a group of at least three structurally and biologically distinct enzymes. Two of them are membrane-bound and have been designated as mPGES-1 and mPGES-2. mPGES-1 is a perinuclear pro...

journal_title：Biochimie

authors： Hara S,Kamei D,Sasaki Y,Tanemoto A,Nakatani Y,Murakami M

更新日期：2010-06-01 00:00:00

abstract：:Phospholipases A(2) (PLA(2)s) and its generation of second messengers play an important role in signal transduction, cell proliferation, cell survival and gene expression. At low concentrations mediators of PLA(2) activity have a variety of physiological effects whereas high levels of PLA(2) and its metabolites are ge...

journal_title：Biochimie

authors： Wang J,Kolko M

更新日期：2010-06-01 00:00:00

abstract：:The distribution of fatty acids among cellular glycerophospholipids is finely regulated by the CoA-dependent acylation of lysophospholipids followed by transacylation reactions. Arachidonic acid is the fatty acid precursor of a wide family of bioactive compounds called the eicosanoids, with key roles in innate immunit...

journal_title：Biochimie

authors： Balgoma D,Montero O,Balboa MA,Balsinde J

更新日期：2010-06-01 00:00:00

abstract：:Phospholipases A2 (PLA2) are a family of enzymes that catalyze the hydrolysis of the sn-2 ester bond of glycerophospholipids liberating lysophospholipids and free fatty acids; important second messengers involved in atherogenesis. Plasma PAF-acetylhydrolase (PAF-AH) or Lp-PLA2 is a Ca(2+)-independent PLA2 which is pro...

journal_title：Biochimie

authors： Karabina SA,Gora S,Atout R,Ninio E

更新日期：2010-06-01 00:00:00

abstract：:LysK, the enzyme lysing cells of Staphylococcus aureus, can be considered as perspective antimicrobial agent. Knowledge of LysK properties and behavior would allow optimizing conditions of its storage as well as formulating strategy towards its stabilization. Reaction of LysK with substrate (suspension of autoclaved S...

journal_title：Biochimie

authors： Filatova LY,Becker SC,Donovan DM,Gladilin AK,Klyachko NL

更新日期：2010-05-01 00:00:00

abstract：:Interleukin-17 (IL-17) is produced exclusively by activated T cells and neutrophils, and stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of "receptor activator of NF-kappaB (RANK) ligand" (RANKL). However, the direct effects of IL-17 on the differentiation of osteoclast precursors in...

journal_title：Biochimie

authors： Kitami S,Tanaka H,Kawato T,Tanabe N,Katono-Tani T,Zhang F,Suzuki N,Yonehara Y,Maeno M

更新日期：2010-04-01 00:00:00

abstract：:Calcium-independent receptor of alpha-latrotoxin (CIRL-1) is an adhesion G protein-coupled receptor implicated in the regulation of exocytosis. CIRL-1 biosynthesis involves constitutive proteolytic processing that takes place in the endoplasmic reticulum, requires the receptor's GPS domain, and yields heterologous two...

journal_title：Biochimie

authors： Deyev IE,Petrenko AG

更新日期：2010-04-01 00:00:00

abstract：:The widespread nature of protein phosphorylation/dephosphorylation underscores its key role in cell signaling metabolism, growth and differentiation. Tyrosine phosphorylation of cytoplasmic proteins is a critical event in the regulation of intracellular signaling pathways activated by external stimuli. An adequate bal...

journal_title：Biochimie

authors： Zambuzzi WF,Milani R,Teti A

更新日期：2010-04-01 00:00:00

abstract：:TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...

journal_title：Biochimie

authors： Bhattacharya M,Biswas A,Das AK

更新日期：2010-03-01 00:00:00

abstract：:Glutaredoxins (Grxs), redox-active proteins with a typical -CPYC motif at their active sites, are involved in redox-regulatory processes and antioxidant defenses. The human malarial parasite Plasmodium falciparum possess a classical glutaredoxin (PfGrx) as well as a number of Grx-like proteins. In the present study, w...

journal_title：Biochimie

authors： Tripathi T,Röseler A,Rahlfs S,Becker K,Bhakuni V

更新日期：2010-03-01 00:00:00

abstract：:The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, re...

journal_title：Biochimie

authors： Attakpa E,Hichami A,Simonin AM,Sansón EG,Dramane KL,Khan NA

更新日期：2009-11-01 00:00:00

abstract：:Latices from Asclepias spp are used in wound healing and the treatment of some digestive disorders. These pharmacological actions have been attributed to the presence of cysteine proteases in these milky latices. Asclepias curassavica (Asclepiadaceae), "scarlet milkweed" is a perennial subshrub native to South America...

journal_title：Biochimie

authors： Obregón WD,Liggieri CS,Trejo SA,Avilés FX,Vairo-Cavalli SE,Priolo NS

更新日期：2009-11-01 00:00:00

abstract：:Long term cadmium (Cd) exposure in occupational and general environments may give rise to kidney dysfunction. This effect is usually considered to be the critical effect, i. e. the effect that occurs at relatively low level of exposure. The present review focused on studies of the prevalence of cadmium-related kidney ...

journal_title：Biochimie

authors： Nordberg GF,Jin T,Wu X,Lu J,Chen L,Lei L,Hong F,Nordberg M

更新日期：2009-10-01 00:00:00

abstract：:Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...

journal_title：Biochimie

authors： Smitha S,Dhananjaya BL,Dinesha R,Srinivas L

更新日期：2009-09-01 00:00:00

abstract：:In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathio...

journal_title：Biochimie

authors： Stefanello FM,Matté C,Pederzolli CD,Kolling J,Mescka CP,Lamers ML,de Assis AM,Perry ML,dos Santos MF,Dutra-Filho CS,Wyse AT

更新日期：2009-08-01 00:00:00

abstract：:Certain transcription factors have recently been shown to interact with DNA in living cells, through very short binding cycles, contrasting with the data previously obtained in vitro, and with the view of a stepwise building of transcription initiation complexes. These short cycles are triggered by active dissociation...

journal_title：Biochimie

authors： Michel D

更新日期：2009-07-01 00:00:00

abstract：:Berardinelli-Seip congenital lipodystrophy (BSCL) is a rare recessive disease characterized by near absence of adipose tissue and severe insulin resistance. In most cases, BSCL is due to loss-of-function mutations in the genes encoding either seipin of unknown function or 1-acyl-glycerol-3-phosphate O-acyltransferase ...

journal_title：Biochimie

authors： Boutet E,El Mourabit H,Prot M,Nemani M,Khallouf E,Colard O,Maurice M,Durand-Schneider AM,Chrétien Y,Grès S,Wolf C,Saulnier-Blache JS,Capeau J,Magré J

更新日期：2009-06-01 00:00:00

abstract：:Our study highlights the tight relationship between protein binding to monolayers and the phase-state of the phospholipids. Interaction of mitochondrial creatine kinase with phospholipidic membranes was analysed using a two-phase monolayer system containing anionic phospholipids under chain mismatch conditions. Monola...

journal_title：Biochimie

authors： Vernoux N,Maniti O,Marcillat O,Vial C,Granjon T

更新日期：2009-06-01 00:00:00

abstract：:Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...

journal_title：Biochimie

authors： Messmer M,Blais SP,Balg C,Chênevert R,Grenier L,Lagüe P,Sauter C,Sissler M,Giegé R,Lapointe J,Florentz C

更新日期：2009-05-01 00:00:00

abstract：:Snake venom from Agkistrodon acutus consists of a number of compounds which may potentially be used as drugs. However, it is hard to obtain enough pure protein for drug development. Recently, we reported expression and purification of a novel recombinant fibrinogenase which was named rFII. Here we reported for the fir...

journal_title：Biochimie

authors： Jiang W,Ma T,Su X,Qiu P,Yan G

更新日期：2009-02-01 00:00:00

abstract：:The biosynthesis of the beta-lactam antibiotic penicillin is an excellent model for the study of secondary metabolites produced by filamentous fungi due to the good background knowledge on the biochemistry and molecular genetics of the beta-lactam producing microorganisms. The three genes (pcbAB, pcbC, penDE) encoding...

journal_title：Biochimie

authors： Kosalková K,García-Estrada C,Ullán RV,Godio RP,Feltrer R,Teijeira F,Mauriz E,Martín JF

更新日期：2009-02-01 00:00:00

abstract：:The innate immunity factor lactoferrin harbours two antimicrobial moieties, lactoferricin and lactoferrampin, situated in close proximity in the N1 domain of the molecule. Most likely they cooperate in many of the beneficial activities of lactoferrin. To investigate whether chimerization of both peptides forms a funct...

journal_title：Biochimie

authors： Bolscher JG,Adão R,Nazmi K,van den Keybus PA,van 't Hof W,Nieuw Amerongen AV,Bastos M,Veerman EC

更新日期：2009-01-01 00:00:00

abstract：:Synthesis and secretion of polysaccharides by Gram-negative bacteria are a result of a concerted action of enzymatic and channel-forming proteins localized in different compartments of the cell. The presented work comprises functional characterization of PssO protein encoded within the previously identified, chromosom...

journal_title：Biochimie

authors： Marczak M,Mazur A,Gruszecki WI,Skorupska A

更新日期：2008-11-01 00:00:00

abstract：:We have cloned a homologue of the translationally controlled tumor protein (TCTP) from the buccal gland of Lampetra japonica according to information from a cDNA library and primary analysis of expressed sequence tags. Sequence analysis of L. japonica TCTP showed that it had two signature regions of high sequence homo...

journal_title：Biochimie

authors： Sun J,Wu Y,Wang J,Ma F,Liu X,Li Q

更新日期：2008-11-01 00:00:00

abstract：:Previous reports on the carbohydrate specificities of Amaranthus caudatus lectin (ACL) and peanut agglutinin (PNA, Arachis hypogea) indicated that they share the same specificity for the Thomsen-Friedenreich (T(alpha), Galbeta1-3GalNAcalpha1-Ser/Thr) glycotope, but differ in monosaccharide binding--GalNAc>Gal (inactiv...

journal_title：Biochimie

authors： Wu AM,Wu JH,Yang Z,Singh T,Goldstein IJ,Sharon N

更新日期：2008-11-01 00:00:00

abstract：:Peroxisomal ABC transporters encoded by the ABCD genes are thought to participate in the import of specific fatty acids in the peroxisomal matrix. ABCD1 deficiency is associated with X-linked adrenoleukodystrophy (X-ALD), the most frequent peroxisomal disorder which is characterized by the accumulation of saturated ve...

journal_title：Biochimie

authors： Leclercq S,Skrzypski J,Courvoisier A,Gondcaille C,Bonnetain F,André A,Chardigny JM,Bellenger S,Bellenger J,Narce M,Savary S

更新日期：2008-10-01 00:00:00

abstract：:Calprotectin (CP) is an abundant protein in human neutrophilic granulocytes and macrophages. In humans, serum, urine, and fecal concentrations of neutrophil-derived proteins, such as CP are used as markers of disease activity for conditions associated with increased neutrophil activity, such as inflammatory bowel dise...

journal_title：Biochimie

authors： Heilmann RM,Suchodolski JS,Steiner JM

更新日期：2008-09-01 00:00:00

abstract：:Bothropstoxin-I (BthTx-I) is a homodimeric Lys49-PLA2 from the venom of the snake Bothrops jararacussu, which lacks hydrolytic activity against phospholipid substrates, yet permeabilizes membranes by a Ca2+-independent mechanism. The interaction of the BthTx-I with model membranes has been studied by intrinsic tryptop...

journal_title：Biochimie

authors： Ferreira TL,Ruller R,Chioato L,Ward RJ

更新日期：2008-09-01 00:00:00

abstract：:Oxazole-containing macrocycles, which include the natural product telomestatin, represent a promising class of anticancer agents that target G-quadruplex DNA. Two synthetic hexaoxazole-containing macrocyclic compounds (HXDV and HXLV-AC) have been characterized with regard to their cytotoxic activities versus human can...

journal_title：Biochimie

authors： Pilch DS,Barbieri CM,Rzuczek SG,Lavoie EJ,Rice JE

更新日期：2008-08-01 00:00:00

abstract：:P53 is a transcription factor also called the "cellular gatekeeper of genome" because it can induce cell cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. Wild type p53 is localised in both the cytoplasm and nucleus of first trimester trophoblastic cells (CTB). Immunoblotting of CTB wi...

journal_title：Biochimie

authors： Cohen M,Wuillemin C,Bischof P

更新日期：2008-06-01 00:00:00

abstract：:In order to study the role of the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK-C) in skeletal muscle, PEPCK-Cmus mice were created by introducing the cDNA for the enzyme, linked to the human alpha-skeletal actin gene promoter, into their germ line. Two founder lines generated by this ...

journal_title：Biochimie

authors： Hanson RW,Hakimi P

更新日期：2008-06-01 00:00:00

abstract：:A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...

journal_title：Biochimie

authors： Mizushina Y,Takeuchi T,Hada T,Maeda N,Sugawara F,Yoshida H,Fujita M

更新日期：2008-06-01 00:00:00